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random primer dna labeling kit  (TaKaRa)


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    Structured Review

    TaKaRa random primer dna labeling kit
    Random Primer Dna Labeling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+primer+dna+labeling+kit/Random+Primer/10__1007_slash_s11627___026___10635___8-99-18-23
    Average 96 stars, based on 6510 article reviews
    random primer dna labeling kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Rad27/FEN1 prevents accumulation of unprocessed Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty ng of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α- 32 P]-dCTP (3,000 Ci/mmol, 10 mCi/mL, Perkin Elmer), using Random Primer DNA Labeling Kit (TaKaRa), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare).

    Article Title: Metal ion homeostasis regulates condensin-dependent chromatin architecture and chromosome segregation in Schizosaccharomyces pombe.
    Article Snippet: .. DNA fragments and plasmid DNA were digested with 4-base cutters, purified by phenol extraction, and labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, PA53021 and PA55021) using a random primer DNA labeling kit (TaKaRa, 6045). .. Images were captured using a Zeiss LSM800 Airy inverted confocal microscope equipped with oil immersion optics (Zeiss, Immersol).

    Article Title: Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes.
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [a-32P]-dCTP (3000 Ci mmol 1, 10 mCi mL 1; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (Cytiva).

    Article Title: Rad27/ FEN 1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α‐ 32 P]‐dCTP (3000 Ci·mmol −1 , 10 mCi·mL −1 ; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer's instructions. .. Unincorporated nucleotides were removed using ProbeQuant G‐50 Micro Columns (Cytiva).

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..

    Polymerase Chain Reaction:

    Article Title: Rad27/FEN1 prevents accumulation of unprocessed Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty ng of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α- 32 P]-dCTP (3,000 Ci/mmol, 10 mCi/mL, Perkin Elmer), using Random Primer DNA Labeling Kit (TaKaRa), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare).

    Article Title: A role for condensin-mediator interaction in mitotic chromosome organization
    Article Snippet: .. PCR products were digested with 5 restriction enzymes (AluI, RsaI, DdeI, HaeIII, and BfuCI) and labeled with Cy3-dCTP (GE HealthCare) or Cy5-dCTP (GE HealthCare) using a random primer DNA labeling kit (Takara). ..

    Article Title: Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes.
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [a-32P]-dCTP (3000 Ci mmol 1, 10 mCi mL 1; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (Cytiva).

    Article Title: Rad27/ FEN 1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α‐ 32 P]‐dCTP (3000 Ci·mmol −1 , 10 mCi·mL −1 ; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer's instructions. .. Unincorporated nucleotides were removed using ProbeQuant G‐50 Micro Columns (Cytiva).

    Random Primer DNA Labeling:

    Article Title: Rad27/FEN1 prevents accumulation of unprocessed Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty ng of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α- 32 P]-dCTP (3,000 Ci/mmol, 10 mCi/mL, Perkin Elmer), using Random Primer DNA Labeling Kit (TaKaRa), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare).

    Article Title: Metal ion homeostasis regulates condensin-dependent chromatin architecture and chromosome segregation in Schizosaccharomyces pombe.
    Article Snippet: .. DNA fragments and plasmid DNA were digested with 4-base cutters, purified by phenol extraction, and labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, PA53021 and PA55021) using a random primer DNA labeling kit (TaKaRa, 6045). .. Images were captured using a Zeiss LSM800 Airy inverted confocal microscope equipped with oil immersion optics (Zeiss, Immersol).

    Article Title: A role for condensin-mediator interaction in mitotic chromosome organization
    Article Snippet: .. PCR products were digested with 5 restriction enzymes (AluI, RsaI, DdeI, HaeIII, and BfuCI) and labeled with Cy3-dCTP (GE HealthCare) or Cy5-dCTP (GE HealthCare) using a random primer DNA labeling kit (Takara). ..

    Article Title: Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes.
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [a-32P]-dCTP (3000 Ci mmol 1, 10 mCi mL 1; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (Cytiva).

    Article Title: Regeneration of fertile transplastomic Arabidopsis thaliana plants from leaves
    Article Snippet: In the model plant Arabidopsis thaliana L., the ability to obtain chloroplast transformants at a high frequency is dependent on transformation of spectinomycin-hypersensitive plant lines, in which the ACC2 nuclear gene was inactivated.. However, when leaves were used for transformation, the regenerated plants were sterile, presumably due to the polyploid nature of the tissue.. To obtain fertile plants at a high frequency, a steroid inducible BABY BOOM (BBM) gene was introduced into the nuclear genome of spectinomycin-hypersensitive Ws-acc2 Arabidopsis plants.

    Article Title: Rad27/ FEN 1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
    Article Snippet: .. Fifty nanogram of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α‐ 32 P]‐dCTP (3000 Ci·mmol −1 , 10 mCi·mL −1 ; Perkin Elmer, Waltham, MA, USA), using Random Primer DNA Labeling Kit (TaKaRa, Shiga, Japan), according to the manufacturer's instructions. .. Unincorporated nucleotides were removed using ProbeQuant G‐50 Micro Columns (Cytiva).

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..

    Plasmid Preparation:

    Article Title: Metal ion homeostasis regulates condensin-dependent chromatin architecture and chromosome segregation in Schizosaccharomyces pombe.
    Article Snippet: .. DNA fragments and plasmid DNA were digested with 4-base cutters, purified by phenol extraction, and labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, PA53021 and PA55021) using a random primer DNA labeling kit (TaKaRa, 6045). .. Images were captured using a Zeiss LSM800 Airy inverted confocal microscope equipped with oil immersion optics (Zeiss, Immersol).

    Extraction:

    Article Title: Metal ion homeostasis regulates condensin-dependent chromatin architecture and chromosome segregation in Schizosaccharomyces pombe.
    Article Snippet: .. DNA fragments and plasmid DNA were digested with 4-base cutters, purified by phenol extraction, and labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, PA53021 and PA55021) using a random primer DNA labeling kit (TaKaRa, 6045). .. Images were captured using a Zeiss LSM800 Airy inverted confocal microscope equipped with oil immersion optics (Zeiss, Immersol).

    Labeling:

    Article Title: Metal ion homeostasis regulates condensin-dependent chromatin architecture and chromosome segregation in Schizosaccharomyces pombe.
    Article Snippet: .. DNA fragments and plasmid DNA were digested with 4-base cutters, purified by phenol extraction, and labeled with Cy3-dCTP or Cy5-dCTP (GE Healthcare, PA53021 and PA55021) using a random primer DNA labeling kit (TaKaRa, 6045). .. Images were captured using a Zeiss LSM800 Airy inverted confocal microscope equipped with oil immersion optics (Zeiss, Immersol).

    Article Title: A role for condensin-mediator interaction in mitotic chromosome organization
    Article Snippet: .. PCR products were digested with 5 restriction enzymes (AluI, RsaI, DdeI, HaeIII, and BfuCI) and labeled with Cy3-dCTP (GE HealthCare) or Cy5-dCTP (GE HealthCare) using a random primer DNA labeling kit (Takara). ..

    Article Title: Regeneration of fertile transplastomic Arabidopsis thaliana plants from leaves
    Article Snippet: In the model plant Arabidopsis thaliana L., the ability to obtain chloroplast transformants at a high frequency is dependent on transformation of spectinomycin-hypersensitive plant lines, in which the ACC2 nuclear gene was inactivated.. However, when leaves were used for transformation, the regenerated plants were sterile, presumably due to the polyploid nature of the tissue.. To obtain fertile plants at a high frequency, a steroid inducible BABY BOOM (BBM) gene was introduced into the nuclear genome of spectinomycin-hypersensitive Ws-acc2 Arabidopsis plants.

    Hybridization:

    Article Title: The histone chaperone CAF-1 prevents homologous recombination-mediated instability of the budding yeast ribosomal DNA during replication-coupled DNA double-strand break repair
    Article Snippet: .. For one Southern hybridization experiment, 50 ng of purified PCR products was used for random priming reactions in the presence of the radiolabeled nucleotide, [α- 32 P]-dCTP (3000 Ci/mmol, 10 mCi/ml, Perkin Elmer), using Random Primer DNA Labeling Kit (TaKaRa), according to the manufacturer’s instructions. .. For Figs , , , and K, and , and Supplementary Fig. , , , and I, 1 μg of gel-purified PCR products were labeled with digoxigenin (DIG)-11-dUTP using DIG-High Prime (Roche), according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..

    Membrane:

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..

    Southern Blot:

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..

    Produced:

    Article Title: PreS1 deletions in genotype C HBV leads to severe hepatic inflammation and hepatocarcinogenesis via the IRE1-JNK axis
    Article Snippet: .. The HBV DNA after purification was loaded on a 1% agarose gel, transferred to a nylon membrane (Hybond N+; Amersham, LC, UK) by Southern blotting, and hybridized with a 32P-labeled wild-type, full- length HBV DNA probe produced with a random primer DNA labeling kit (TaKaRa, Tokyo, Japan). ..



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